Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system
نویسندگان
چکیده
منابع مشابه
Mind the Organelle Gap - Peroxisome Contact Sites in Disease.
The eukaryotic cell is organized as a complex grid system where membrane-bound cellular compartments, organelles, must be localized to the right place at the right time. One way to facilitate correct organelle localization and organelle cooperation is through membrane contact sites, areas of close proximity between two organelles that are bridged by protein/lipid complexes. It is now clear that...
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Since their initial observation, contact sites formed between different organelles have transitioned from ignored curiosities to recognized centers for the exchange of metabolites and lipids. Contact formed between the ER and endomembrane system (eg. the plasma membrane, endosomes, and lysosomes) is of particular biomedical interest, as it governs aspects of lipid metabolism, organelle identity...
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1 Anatomy and Developmental Biology Program, Institute of Biomedical Sciences, University of Chile, Santiago, Chile, 2 Geroscience Center for Brain Health and Metabolism, Santiago, Chile, 3 Buck Institute for Research on Aging, Novato, CA, United States, 4 Department of Chemistry and Biochemistry, University of California, Santa Barbara, Santa Barbara, CA, United States, 5 Department of Morphol...
متن کاملDouble labelling of subcellular structures with organelle-targeted GFP mutants in vivo
BACKGROUND The green fluorescent protein (GFP) of Aequorea victoria is emerging as a unique tool for monitoring complex phenomena such as gene expression and organelle structure and dynamics in living cells. The recent description of GFP mutants with modified spectral properties opens numerous new applications in cell biology. However, the expression and the characteristics of these GFP mutants...
متن کاملOrganelle pH studies using targeted avidin and fluorescein-biotin.
BACKGROUND Mammalian organelles of the secretory pathway are of differing pH. The pH values form a decreasing gradient: the endoplasmic reticulum (ER) is nearly neutral, the Golgi is mildly acidic and the secretory granules are more acidic still ( approximately pH 5). The mechanisms that regulate pH in these organelles are still unknown. RESULTS Using a novel method, we tested whether differe...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2018
ISSN: 2045-2322
DOI: 10.1038/s41598-018-24466-0